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KMID : 0350519920450020715
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Journal of Catholic Medical College
1992 Volume.45 No. 2 p.715 ~ p.733
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DNA Proliferative Variables and P-Glycoprotein Expression in Human Ovarian Carcinomas and their Relationships to Pathological Parameters
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Abstract
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Paraffin embedded tumor samples form 77 ovarian tumor patients were analyzed by DNA flow cytometry and immunoperoxidase staining(labelled streptavidin-biotin method) fro P-glycoprotein expression. The ovarian carcinomas were composed of 57
carcinomas
and 20 tumors of borderline malignancy. Two carcinomas in DNA analysis and one carcinoma in stain processing for P-glycoprotein were excluded due to unsatisfactory results.
Ten normal ovaries were used ass control group in DNA analysis and marrow tissue sections from three acute monocytic leukemia patients were used as control group in immunoperoxidase staining.
DNA proliferative fractions were calculated by bitmap gating using flow cytometry in S, G2, M phases and aneuploidy was determined from the DNA histogram using linear S-phase method, and P-glycoprotein which expressed by ovarian carcinomas was
studied
by immunoperoxidase staining. Possible relationships between these two indices(DNA proliferative variables and P-glycoprotein expression rate) and a number of pathological parameters were investigated in the ovarian carcinomas. The pathological
parameters assessed were cell types, mitotic index, histological grade, nuclear grade, tumor necrosis, cellular reaction and tumor size. DNA proliferative fractions and aneuploidy as DNA proliferative variables were chosen.
@ES The results were as follows:
@EN 1. The proliferative fractions of ovarian carcinomas showed a mean of 24.98%(SD 10.84%, range 3.36-62.26%).
2. Of 55 carcinomas, 20 were aneuploidy and 35 diploidy. Of 20 borderline malignant tumors. 4 showed aneuploidy and 16 diploidy. The aneuploidy was noted in 47.8% of histological grade III carcinomas and 50.5% of grade II carcinomas, and none in
the
grade I carcinomas.
3. DNA aneuploidy signficantly increased in histological grade II and III compared with grade I (X*=10.7, P=0.001), but there was no signficant difference between grade II and III.
4. There was a positive correlation between DNA proliferative fractions and mitotic index(r=0.58 P=0.0001). There was a weak correlation between proliferative fractions and histological grade(r=0.40, P=0.002), and a very weak correlation between
proliferative fractions and nuclear grade(r=0.28, P=0.04). There were also significant differences among grade I, II, III in the mitotic index(P=0.0001) and the histological group(P=0.0015). Mitotic index grade II and III significantly
increased(P<0.01)
compared with grade I, but no difference was noted between grade II and III. Histological grade II and III significantly increased in tumor growing(P<0.01, P<0.05) compared with grade I, but no difference was noted between grade II and III. There
was no
significant difference between proliferative fractions and the other parameters such as tumor nercrosis, cellular reaction and tumor size.
5. P-glycoprotein expression was noted only in the mucinous and seruous tumor groups. These groups signficantly incresed in expression for P-glycoprotein compared to remaining carinomas(X*=6.6, P=0.04).
6. There was a negative correlation between P-glycoprotein expression rate and histolgoical grade (X*=6.3, P<0.05). Positive stainings of P-glycoprotein expression by carcinoma cells were in 38.5% of histological grade I carcinomas, 33.3% of
grade
II
carcinomas and 5.0% of grade III carcinomas, respectively. The expression rate was higher in the lower histological grade among grade I, II and III. There was no significant difference between P-glycoprotein expression rate and the other
pathological
parmeters such as mitotic index, nuclear grade, tumor necrosis, cellular roaction, and tumor size.
7. There were no differences between P-glycoprotein expression and proliferative fractions, and between P-glycoprotein expression and aneuploidy.
In conclusion, no strong correlation was noted between DNA proliferative fractions and pathological parameters, although mitotic index, histological and nuclear grades showed signifcant differences compared with DNA proliferative fractions. This
suggests that DNA proliferative fractions and aneuploidy can serve as useful pathological indicators to cell proliferation assessment of ovarian carcinomas, and P-glycoprotein expression may be related to the proliferative sctivity of mucinous
and
serous carcinoma groups.
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